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Sara Carillo | Authors
Articles
Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest
Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
High-Throughput Peptide Mapping of Monoclonal Antibodies Using Tandem Liquid Chromatography– Mass Spectrometry
The benefits of tandem LC–MS are revealed.
Multi-Attribute Monitoring and the Multi-Attribute Method: A Powerful Double Act for Supporting Biopharmaceutical Manufacturing
This work compares at-line Protein A-MS with a standard multi-attribute method (MAM) workflow for analyzing product quality attributes (PQAs) during upstream processing.
Exploring Capillary Electrophoresis–Mass Spectrometry for the In-Depth Intact Native Analysis of Cysteine-Conjugated Antibody-Drug Conjugates
This article explores the potential of microchip capillary electrophoresis–mass spectrometry (CE–MS) for the characterization of intact biopharmaceuticals, and how this technique can effectively be employed to monitor essential quality attributes of interest.