LCGC International’s Content Peaks from 2025

In 2025, LCGC International remained committed to delivering authoritative troubleshooting guidance and practical application solutions across all areas of separation science. Our content is designed to support laboratory-based analytical chemists by strengthening practical knowledge, accelerating problem-solving, and enabling informed decision-making in increasingly complex analytical environments.

Guided by rigorous scientific criteria, commercial objectivity, and real-world relevance, LCGC International equips its readers with trusted tools to address everyday analytical challenges—enhancing efficiency, productivity, and competitive advantage in the modern laboratory. The key to the success of LCGC International is authoritative insights provided and assessed by our active editorial advisory board (EAB) and key opinion leaders in separation science.

Practical, expert advice remains a core component of the information we provide, and our most highly sought-after content—delivered across a range of print and digital formats—is closely aligned with this practical, problem-solving mission statement.

LCGC International: Providing Practical Information From Experts You Can Trust
Among the many highlights from LCGC International in 2025, “LC Troubleshooting” editor Dwight Stoll explored advanced oligonucleotide analysis techniques, focusing on chromatography methods for therapeutic RNA, siRNA, and mRNA. His article, Challenges and Solutions in Oligonucleotide Analysis: An Overview of Liquid Chromatography Methods and Applications, provided valuable insight into strategies that are advancing “oligo” research.

Analytically Speaking
Stoll’s “Analytically Speaking” podcast, co-hosted with Jim Grinias, continues to resonate strongly with our audience. In February, they reviewed highlights from Pittcon 2025 from a separation science perspective, joined by Katelynn Perrault Uptmor and Emanuela Gionfriddo. The discussion offered an illuminating look at emerging trends and innovations in the field. Listen here.

Green Chemistry and Sustainability in Analytical Science
With growing interest in green chemistry and sustainability, analytical chemists are reevaluating methods with increased emphasis on environmental impact. Since the introduction of green chemistry and green analytical chemistry principles, several tools have emerged to assess method sustainability. Three widely used metrics—Red-Green-Blue (RGB), AGREE, and the Analytical Method Greenness Score (AMGS)—can be applied to gas chromatography (GC), demonstrating that classical GC remains one of the greenest analytical techniques.

This topic is explored in our November/December cover story by “GC Connections” editor Nicholas Snow, recipient of the 2025 EAS Award for Outstanding Achievements in Separation Science, in an article entitled: How Green Is Your Method? Evaluating the Sustainability of Analytical Methods

HPLC 2025 Review and Advances in Stationary Phases
“Column Watch” editor Dave Bell continues to provide readers with authoritative updates on the evolution and practical use of stationary phases. His annual HPLC 2025 review remains a must-read in the chromatography content calendar. Bell also collaborated with a team from Apple to offer a unique perspective on per- and polyfluoroalkyl substances (PFAS) analysis in the article: Toward Better Detection of PFAS in Consumer Electronic Products: Optimizing the Accuracy of a Total Fluorine Test Method in Materials Containing Polymeric PFAS. Combustion ion chromatography (CIC) is a promising technique for analyzing PFAS presence and is becoming more commonly used in PFAS analysis.

Quality, AI, and the “Hidden Factory”
There is a hidden factory in plain sight in many laboratories—rarely noticed, yet ever-present. What does this hidden factory produce, and how does it affect your work? These questions are explored in Bob McDowall’s “Question of Quality” column: The Hidden Factory in Your Laboratory.

McDowall also tackles one of the most pressing questions of the year in The Answer’s AI. What’s the Question? He examines whether artificial intelligence (AI) and machine learning (ML) truly enhance chromatographic peak integration, what the regulators are saying, and whether analysts risk being seduced by technology rather than guided by sound science.

Advances in Multidimensional Chromatography in Biopharmaceutical Analysis
The development of two-dimensional liquid chromatography (2D-LC) continues to open new opportunities for innovation in instrumentation, automation, and data analysis. In “Focus on Biopharmaceutical Analysis,” Anurag S. Rathore, Debasmita Chakraborty, and Jared Auclair review the role of 2D-LC applications in biopharmaceutical analysis from 2018 to 2024 in the article: Two-Dimensional Liquid Chromatography Applications in Biopharmaceutical Analysis.

MAPPING The Territory/HILIC
In our July/August cover story from “Biopharmaceutical Perspectives,” Koen Sandra highlighted novel research on MHC-Associated Peptide Proteomics (MAPPs) as a tool for assessing the immunogenicity risk of therapeutic monoclonal antibodies. The study demonstrated that MAPPs is a practical, sensitive, and informative method for evaluating immunogenicity potential. In March 2025, a team from Waters explored: Hydrophilic Interaction Liquid Chromatography for Oligonucleotide Therapeutics: Method Considerations. Further research into the retention mechanisms of oligos using HILIC could further enhance the robustness and applicability of this technique in this rapidly evolving field.

Back to Basics: Data Description and Statistics
Bob Pirok returned to fundamentals in his column, How to Meaningfully Describe and Display Analytical Data? A Dive into Descriptive Statistics. In this installment, Pirok revisits the robustness of the standard deviation, using both statistical theory and visual tools to help readers better understand and communicate analytical data.

Advances in (U)HPLC
Active EAB member, Gert Desmet from the Vrije Universiteit, Brussels, Belgium, guest-edited a special supplement related to HPLC 2025—Advances in (U)HPLC—with contributions from key speakers at the event. Topics included a combined iterative DDA–DIA mass-spectrometry workflow, the use of LC×SFC orthogonal 2D separations for complex samples, the role of exposomics in food-safety applications, non-animal approaches to predicting skin permeability using LC and SFC, the development of 3D-printed sorbents for untargeted LC–QTOF-MS, and high-throughput metabolomics using dried blood spots (DBS).

Virtual Symposium Series: The Keys to Successful Separation Science in Practice
An essential component of delivering practical advice to our audience is LCGC International’s successful virtual symposiums, where experts in the practical art of separation science provide the key to successful separations. It’s not too late to catch these symposiums on demand!

PFAS Summit 2025
Building on the success of LCGC’s previous PFAS Summit in 2024, LCGC International broadcast another successful, interactive, two-day virtual symposium aimed at laboratory scientists specializing in the analysis of PFAS and who need to keep abreast of the latest chromatographic advances. The LCGC International PFAS Summit 2025 focused on the latest techniques and best practices for analyzing PFAS in food and environmental samples. View on-demand here.

ChromTalks 2025: Essential Troubleshooting Tips and Tricks
This year, the focus of our first ChromTalks symposium aimed to help you troubleshoot your liquid chromatography (LC) and gas chromatography (GC) workflows through all stages to develop an effective “trouble-free” chromatographic method. Leading practitioners provided both specific troubleshooting tips, as well as an explanation of the underlying principles to enable you to make the best choices for your separation. It’s not too late to brush up on your skills. View on-demand here.

Advances in Gas Chromatography: A Virtual Symposium
LCGC International, in collaboration with the Chromatographic Society (ChromSoc), presented a one-day virtual symposium focusing on recent advances in GC. The first session included a series of presentations from leading practitioners in the field, followed by an interactive question-and-answer (Q&A) session with the speakers to address audience questions. In the afternoon, leading instrument suppliers discussed how their GC technologies could help you perform more effectively in practice. View on-demand here.

Separation Science—The State of The Art in Life Science Analysis: A Virtual Symposium
Separation scientists play a pivotal role in the development of innovative therapeutics that benefit human health and society. In this special two-day symposium, again organized by LCGC International in collaboration with ChromSoc), leading practitioners in the field discussed the latest cutting-edge trends and technologies used for both small-molecule and large-molecule analysis. View on-demand here.

ChromTalks 2025: Mastering Hyphenated Method Development
The second successful ChromTalks symposium of the year focused on Mastering Hyphenated Method Development. The event was organized by LCGC International in collaboration with CHROMacademy and the HTC-19 Scientific Committee. Through engaging tutorial presentations and interactive live Q&A sessions, attendees gained practical, real-world strategies for optimizing method development across complex, hyphenated platforms. Attendees left with actionable insights to streamline workflows, improve reproducibility, and enhance analytical performance. View on-demand here.

Peer Exchange™ Panel Discussions
Our innovative “peer exchange” video panel discussion series provided a platform where leading chromatography practitioners shared their insights, challenges, and experiences. These sessions encourage open dialogue and collaborative problem-solving in key application areas.

Oligonucleotide Analysis: A Separation Science Perspective
Michael Bartlett, professor of pharmaceutical and biomedical sciences at the University of Georgia, Athens, Georgia, USA, moderated the discussion with leading experts in oligonucleotide analysis: Torgny Fornstedt, professor in analytical chemistry and leader of the Fundamental Separation Science Group, Karlstadt University, Karlstadt, Sweden, and Mark Dickman, professor in bioanalytical science and engineering at the University of Sheffield, Sheffield, UK. View here.

Driving Progress in PFAS Analysis
Moderated by Rainer Lohmann, professor of oceanography, marine and atmospheric chemistry at the University of Rhode Island, USA, the discussion included Carrie McDonough, assistant professor of chemistry at Carnegie Mellon University, USA; Natalia Soares Quinete, assistant professor of chemistry and biochemistry at Florida International University, USA; and Stefan van Leeuwen, senior scientist at Wageningen Food Safety Research, Wageningen University & Research, Netherlands. Together, these scientists set the stage for a robust exploration of the analytical and regulatory challenges in PFAS research. Watch the full series of videos from this peer exchange here.

GLP-1 Therapies in Transition: Analytical and Clinical Insights for Experts
In today’s rapidly evolving therapeutic landscape, glucagon-like peptide-1 receptor agonists (GLP-1 RAs) have emerged as a transformative class of drugs, reshaping the management of metabolic diseases and expanding into new clinical applications. In a recent LCGC International Peer Exchange™, we spoke with two experts at the forefront of GLP-1 research and clinical practice: Judith Hey-Hadavi, vice president of Medical Affairs at Worldwide Clinical Trials, and Nicole Snow, clinical pharmacist at Olympia Pharmaceuticals, to explore forward-looking insights and emerging trends in this field. Visit the main page here.

Supplements
Supplements from LCGC International in 2025 included Advances in. Biopharmaceutical Analysis, Hot Topics in Gas Chromatography,Current Trends in Mass Spectrometry,Advances in (U)HPLC, Hot Topics in Mass Spectrometry, and Hot Topics in PFAS Analysis.

News, Views, and Interviews
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