Stephen Ball

This article discusses what recent advances in gel permeation chromatography/size-exclusion chromatography (GPC/SEC) can deliver in polymer development.

Using GPC/SEC for Excipient Characterization

This article explores how multiple detectors, particularly light scattering detectors, may be used for size-exclusion chromatography (SEC) in protein analysis.

Introduction

Bradykinin, a 9 amino acid peptide, is a physiologically and pharmacologically active peptide of the kinin group of proteins, which is used in the development of antagonists and therapies for hereditary angioedema. In this application, 50 mg of crude bradykinin, synthesised on a StratoSpheresâ„¢ PL-Rink resin, is purified using an HPLC method that can be scaled from the laboratory through to full production.