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© 2022 MJH Life Sciences™ and Chromatography Online. All rights reserved.
In situ measurements of the mobile-phase pH before and after the column help to rationalize the effects of mismatch in pH and concentration between the mobile phase and sample buffer mismatch in reversed-phase LC separations.
If I inject a sample buffered at a pH different from that of the mobile phase, how quickly is the sample buffer neutralized inside the column?
We return to the important topic of buffers, this time focusing on what happens when there is a mismatch between the mobile-phase buffer pH and the pH that the sample is buffered at.
What concentration of aqueous buffer should I use in the mobile phase when developing a re-versed‑phase liquid chromatography (LC) method for the analysis of ionogenic compounds?