In this first installment in a series on the causes of peak asymmetry, we discuss basic concepts in peak shape, explore poor fluidic connections as a common cause of peak tailing, and explain what to do about it.
In recent articles, the authors reviewed the basic concepts of extracolumn dispersion and how this phenomenon can impact the quality of an LC separation. We now specifically discuss the effects of dispersion that can occur due to tubing and detectors.
Dispersion of analyte peaks outside of chromatography columns can seriously erode the resolution provided by good columns. Here, we focus on the contribution of the sample injection step to the total level of extracolumn dispersion in an LC system.