Dwight R. Stoll | Authors

When can analyte retention deviate from what is expected or normal? We explain three subtle causes.

Even relatively simple mixtures are not always easy to separate. What are the options for adjusting selectivity in reversed-phase LC separations?

In this latest instalment of our series on recurring challenges in liquid chromatography (LC), we turn our attention to peak widths that are wider than expected. Estimating the expected peak widths can help determine when a column and LC system are not working quite right, and to decide when troubleshooting should be initiated.

When considering different ways to improve an established LC method, it helps to start by reviewing some essential concepts.

Developing a short list of the likely causes of these results can help streamline our troubleshooting experience when sensitivity-related problems occur.

Estimating expected peak widths helps us determine when a column and LC system is not performing optimally.