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The Application Notebook-02-01-2014

The Application Notebook

Analysis of Coffee and Coffee Products by HPLC for the Determination of Natural Components and Harmful Contamination

February 01, 2014

Food and Beverage

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In this application we demonstrate the analysis of coffee and coffee products according to DIN methods. The performance of the analysis is shown for linearity, limit of detection (LOD), limit of quantification (LOQ), retention time, and area precision.

Molecular Weight Determination of Unfractionated Heparin

February 01, 2014

Medical/Biological

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Heparin is a polydisperse, heterogeneous polysaccharide derived from animal tissue. Heparin has been used as an anticoagulant for over 60 years and one of the fundamental parameters for characterizing unfractionated heparin (UFH) is its molecular weight (MW) and MW distribution.

Hexavalent Chromium Determination Hamilton PRP-X100 Anion Exchange HPLC Column

February 01, 2014

Environmental

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Chromium (Cr) is a metal with an interesting relationship to the environment. Whereas trivalent chromium (Cr(III)) is an essential nutrient, hexavalent chromium (Cr(VI)) is a poison to humans and aquatic life and poses serious environmental and ecological threats.

Optimizing LC–MS and LC–MS-MS Methods

February 01, 2014

Articles

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Many parameters within liquid chromatography–mass spectrometry (LC–MS) methods are "locked and left" - that is, they are optimized using a few methods when new, then only changed if absolutely necessary because of poor analytical performance.

The Automated Extraction of Aqueous Samples by Method EPA 8270D Using the TurboTrace ABN SPE System

February 01, 2014

Environmental

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EPA 8270D calls for the extraction for analysis of semi-volatile analytes in various matrices. Target analytes mentioned in the method cover a wide range of compound classes resulting in reporting lists that often approach 100 compounds.

Modernizing the USP Monograph for Acetaminophen

February 01, 2014

Pharmaceutical/Drug Discovery

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In the USP method for acetaminophen (1), the related compound p-aminophenol is determined using spectrophotometry while another related compound, p-chloroacetanilide, is determined using TLC.

Vol 32 No 2 LCGC North America February 2014 Application Notebook PDF

February 01, 2014

Issue PDF

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Click the title above to open the LCGC North America February 2014 Application Notebook, Vol 32 No s2, in an interactive PDF format.