Analysis of Limonin in Citrus Juice Using QuEChERS and LC–MS/MS
March 01, 2017
This application note outlines a simple, fast, and cost‑effective QuEChERS-based method for the determination of limonin in citrus juice. Limonin is extracted from a variety of juice samples using acetonitrile and citrate-buffered salts. The sample extract undergoes cleanup by dispersive-SPE (dSPE) using primary‑secondary amine (PSA), C18, and graphitized carbon black (GCB) to remove unwanted matrix components, including sugars, acids, and pigments, and to yield a clear sample extract. Analysis is performed by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) using a Selectra® C18 HPLC column (although high performance liquid chromatography [HPLC]–UV can also be used).