Gesa J. Schad | Authors

Articles

Advanced Peak Processing to Reduce Efforts in Method Optimization

Partial coelution of chromatographic peaks is an often-encountered issue in high performance liquid chromatography (HPLC) analysis, despite best efforts in method development and optimization. Even though there are several ways of integration of overlapping signals, accurate quantification of single compounds using conventional photodiode array (PDA) detection is almost impossible without baseline separation. While extensive signal processing is well established in spectroscopic analyses such as infrared (IR) or nuclear magnetic resonance (NMR), it has not yet been commonly adapted to improve chromatographic data evaluation. This article introduces the theory and application of a novel data analysis technique for PDA detection to accurately determine and quantify single compounds, even from overlapping peaks, without the need for mass spectrometry (MS) detection.

Determination of Cannabidiol and Additional Cannabinoid Content in Hemp Tea

Quantification of cannabinoids is essential for the accurate labelling of hemp products, both for quality control and for establishing legality with regards to d9-tetrahydrocannabinol (THC) content. For this article, the cannabinoid content of several cannabidiol (CBD)-rich hemp tea samples was determined and was found to often deviate from the content stated on the packaging. Just like the cannabis flower, hemp tea can be analyzed easily and effectively for its cannabinoid content using high performance liquid chromatography (HPLC). The HPLC–UV assay of choice provided good linearity, low limits of detection (LODs), and high precision of retention time and peak area for the 11 cannabinoids under investigation.

Fast and Simple High Performance Liquid Chromatography–Ultraviolet Assay for the Determination of Cannabinoid Content in Hemp Oil

The medicinal qualities of cannabinoids contained in hemp have been described in detail. Pain mitigation and reduced severity of nausea and seizures are just a few of the therapeutic benefits reported by medical cannabis patients. There is evidence that a combination of CBD, a host of other minor cannabinoids, and a complex array of terpenoids as contained in hemp oil may be the most beneficial, which is why CBD-rich oil for oral intake has become increasingly popular. The FDA has issued warning letters to firms that market unapproved new drugs allegedly containing CBD. As part of these actions, the cannabinoid content of some hemp products was determined and many were found to contain levels of CBD largely deviating from the label claim. Like cannabis, hemp oil can be analyzed easily and effectively for its cannabinoid content. This article highlights the use of a fast and simple HPLC–UV assay for separation and quantification of 11 important cannabinoids, including CBD in hemp oil.

Fast and Sensitive Analysis of Amino Acids Using Automated Pre-Column Derivatization

The building blocks of peptides and proteins, amino acids, are present in animals, humans, and plants. The analysis of amino acids is of essential significance in several areas including food science, clinical diagnostics, and pharmaceutical products research. Classical liquid chromatography (LC) determination of amino acids is performed with sample pretreatment including pre-column as well as post-column derivatization to improve or enable spectroscopic detection.