The Application Notebook-09-01-2010

Site-specific analysis of protein glycosylation has become a very important issue in pharmaceutical/biotech industries, requiring specific analytical tools and methodologies.

Urine is often used to analyze drugs of abuse because it is easy to collect and is available in copious amounts.

Aflatoxins occur naturally in peanuts, cottonseed, corn, and dried chili pepper, as well as many mixed or processed foods and feeds.

Method development in reversed-phase liquid chromatography (RPLC) can be challenging for structurally similar compounds.

Electrochemical detection (ECD) is both selective and extremely sensitive. However, relatively few compounds are electrochemically active, therefore limiting its application.

Complete high resolution cross-fractionation of Polyolefins in the molar mass and composition dimensions is achieved with a new benchtop CFC analytical instrument.

Methods: Vials and closures were exposed to acetonitrile for 2 h and subsequently analysed by LC-UV, LC–MS, and GC–MS to characterize the interferences.

Whisky is a complex and variable material, the quality of which is affected by the raw materials and processing.

Gel-permeation chromatography (GPC) is a highly effective postextraction cleanup method for removing high molecular-weight interferences such as lipids, proteins, and pigments from sample extracts prior to analysis.

P-hydroxybenzoic acid esters, also called parabens, are widely used as antimicrobial preservatives in cosmetic products.

Modern analytical applications often demand definitive tandem MS results on ever more complex samples utilizing fast separation techniques.

The QuEChERS approach is used for the extraction of 16 PAH compounds from fish with analysis by LC using fluorescence detection.

Since the addition of electron transfer dissociation (ETD) to the Thermo Scientific LTQ Orbitrap hybrid mass spectrometers, ETD has become a standard tool for proteomics research together with the traditional collision-induced dissociation (CID) (1).

For many years, the use of guard columns has been advocated by column manufacturers and other experts to protect and extend column lifetime and performance of analytical columns from potential damage caused by the presence of chemical contaminants and microparticulates in the sample and mobile phase.

Click the title above to open the LCGC North America September 2010 Application Notebook, Vol 28 No s9, in an interactive PDF format.