The Application Notebook-09-01-2010

Cationic surfactants are used as disinfectants, corrosion inhibitors, phase-transfer catalysts, wetting agents, dispersants, and even mobile phase modifiers in liquid chromatography.

ISOLUTE EPH columns have been developed to fractionate pentane or hexane based soil extracts into aliphatic and aromatic hydrocarbon fractions (C8?C40 aliphatics, C10?C22 aromatics).

Viruses are packets of infectious nucleic acid (either DNA or RNA) surrounded by a protective coat consisting of a large number of protein subunits.

Cocaine is a central nervous system stimulant derived from Erythroxylon coca.

Protein-based pharmaceutical drugs, such as fusion proteins and monoclonal antibodies (mAb), are playing an increasing role in modern therapeutics.

Polysaccharide-based chiral stationary phases (CSPs) are often utilized for a broad range of enantiomeric chromatographic separations due to their wide chiral recognition ability.

Furan is naturally occurring at low levels in many foods and drinks (1).

The new MAbPacTM SCX-10 column is based on 10 µm nonporous, highly cross-linked styrene-type polymeric media with a proprietary uniform hydrophilic coating that is grafted with sulfonic acid functional groups.

Chemical modification of therapeutic proteins in order to enhance their biological activity is of increasing interest.

The Chirosil RCA(+) and SCA(–) chiral stationary phases (CSPs) are specialized chiral phases very effective in separating various natural and unnatural amino acids as well as compounds containing primary amines.

An extension of ASTM D7169 is shown using new Zebron ZB-1XT SimDist metal GC columns.

In developing the Atomx automated sample prep system, Teledyne Tekmar integrated both a P&T concentrator and a multi-matrix autosampler into a single platform system that can be used in the analysis of soil, water, and soils requiring methanol extraction.

Illicit cocaine, amphetamine, methamphetamine, and ecstasy (MDMA) are often encountered in the form of salts and identification of the specific counter ion present may provide valuable information about the source of production and the manufacturing pathway.

Hydroxyethylstarches (HES) are used increasingly as plasma expanders in medical applications.

Coffees have aroma and taste based on where the beans are grown, roasting techniques, and flavor additives.

This note examines the benefits of using flash or HPLC for compound purification. Results show that flash is a viable alternative to HPLC.

Burning mosquito coils to repel these pests is common in many countries; however the emission of various, potentially toxic volatile organic compounds (VOCs) is cause for concern.

N-Nitrosodimethylamine and other structurally similar nitrosamines are classified as probable carcinogens by the U.S. EPA, and their presence has been reported in California drinking water since 1998.

Levoglucosan is a combustion product of cellulose and, as such, widely used as a unique tracer for biomass burning sources in atmospheric aerosols.

Agilent Poroshell 120, a superficially porous particle column, offers resolution and speed similar to columns packed with 1.8-µm totally porous particles, without generating high back pressure.

Gel filtration chromatography is the primary method used to analyze the amount of aggregate and dimer present in a therapeutic protein sample.

This paper describes the use of the AB SCIEX TripleTOFTM 5600 LC–MS-MS system for targeted and nontargeted screening for pesticide residue in food samples.

Fast, flexible platforms for peptide quantification are needed, particularly for a discovery setting.

LCGC is planning to publish the next issue of The Application Notebook special supplement in December.

The optical isomers of flavanone have been separated using the EpitomizeTM CSP-1 series of chiral stationary phases.

This article reports a new sample injection mechanism for an autosampler that directly transfers a sample to column without passing it through any flow-changing device, and hence minimizes a delay volume and reduces a carryover signal to the extreme level.

In this application note we present an online SPE-LC method for the determination of trace-level endocrine disruptive compounds (EDCs) in seawater using the new SolEx HRP cartridge as the solid-phase extraction (SPE) phase.

A sample of ambient cabin air from a commercial airliner was collected by stir bar sorption extraction (SBSE) and analyzed by thermal desorption GCxGC-TOFMS.

To the members of LCGC's readership, the science behind the headlines is always apparent. Separation science in general, and chromatography in particular, find application in nearly every facet of everyday life, from pharmaceutical analysis to the biomedical field to environmental testing, and this Application Notebook is a tribute to just how thoroughly separation science permeates our daily lives.

The new MAbPacTM size exclusion (SEC) column is based on high-purity, spherical, porous (300 Å), 5 µm silica covalently modified with a proprietary diol hydrophilic layer.