The Application Notebook-10-02-2010

i4-697763-1408634828019.jpg

The Application Notebook

In recent years water-soluble polymers are gaining more and more interest in different applications. The molecular weight distribution of polymers is usually characterized by size exclusion chromatography (SEC) coupled with refractive index, viscometric or laser light scattering detection. Recent advances in SEC comprise semi-micro SEC and the design of linear columns providing wide molecular weight separation ranges and near-linear calibrations. We describe the separation of water-soluble polymers with a new generation of linear, polymer-based, semi-micro SEC columns using the compact EcoSEC SEC system.

i4-697749-1408634855280.jpg

The Application Notebook

A robust, selective and sensitive separation method for the determination of four steviol glycosides in stevia plant matrix is demonstrated. In addition to the two calibrated substances two more glycosides could be found in stevia plant extracts by LC–MS. Using a popular Eurospher NH2 phase and applying moderate separation conditions results in an inexpensive and precise analysis method.