LCGC Asia Pacific-03-01-2015

There has been a revival of supercritical fluid chromatography (SFC) in recent years, especially in the chiral preparative field, but also more recently in the analytical area. However, SFC is considerably more complex than liquid chromatography (LC), mainly because of the compressibility of the mobile phase. One can say that SFC is a “rubber variant” of LC where everything considered constant in LC varies in SFC. In this review, we go through advances in theory, instrumentation, and novel applications.

A stepwise process is described to help isolate and identify the cause of a method failure.

31st International Symposium on MicroScale Bioseparations

The numerous advantages of translating gradient chromatographic methods between the differing formats of LC have been explored and discussed. While translations in principle obey well defined chromatographic theories, the authors investigate a number of potential pitfalls that may result in poor translations as exhibited by selectivity differences, changes in efficiency, and hence failure to meet resolution system suitability criteria.

The transit of peaks through a gas chromatography (GC) column depends strongly on the thermal profile they encounter on the way. Gas chromatographers primarily rely on the classic hot?air?bath type of oven, but several alternatives are also in use. This instalment examines ovens for GC in several forms plus how oven thermals affect peak retention behaviour.

Click the title above to open the LCGC Asia Pacific March 2015 regular issue, Vol 18, No 1, in an interactive PDF format.