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Patrik Petersson | Authors
Articles
Anomalous Retention Prediction Using Modelling Software in Gradient Reversed-Phase Liquid Chromatography: Why it Can Occur and How to Prevent It
The success of screening column and mobile phase combinations that generate dissimilar selectivity is highlighted in a typical method development strategy.
Method Development for Reversed-Phase Separations of Peptides: A Rational Screening Strategy for Column and Mobile Phase Combinations with Complementary Selectivity
This review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.
Dealing with Wandering First-Dimension Peaks in 2D-LC Separations
Retention time fluctuations are challenging to deal with, especially in two-dimensional liquid chromatography (2D‑LC) separations. Here, two approaches are presented that can help navigate this challenge.
Dealing with Wandering First-Dimension Peaks in 2D-LC Separations
Retention time fluctuations are challenging to deal with, especially in two-dimensional liquid chromatography (2D-LC) separations. Here, two approaches are presented that can help navigate this challenge.
Fluctuating Peptide Retention in 2D-LC: How to Address a Moving Target
In this installment, tips, tricks, and suggestions are provided for best practices in reversed-phase separations, in both 1D and 2D LC, with gradient elution for minimum variations in retention time.
Understanding How Dwell Volume Can Affect Selectivity in Reversed-Phase Gradient Chromatography
The effect of dwell volume on chromatographic selectivity can be successfully modelled using retention prediction software. Hence, the robustness of reversed-phase LC gradient methodologies, with respect to dwell volume, can be conveniently assessed.