Retention and Selectivity of Stationary Phases Used in HILIC
November 01, 2015
Hydrophilic-interaction chromatography (HILIC) is a complex system involving partition, polar, and ion‑exchange interactions. Method development can be greatly facilitated by understanding the interactions that the different stationary phases provide and applying that knowledge to the separation task at hand. Through an understanding of the main differences of the set of analytes to be separated, a column or set of columns can be judiciously chosen to screen for effective retention and selectivity.