A Simple and Interactive Column Classification for Reversed-Phase Liquid Chromatography: The Carotenoid Test, Part 1: Studied Properties, Probes, and Fundamentals
October 01, 2016
Cartenoid compounds can be used as probes for studying bonded stationary phases for reversed-phase liquid chromatography, such as C18, phenyl-hexyl, and cholesteryl. From one supercritical fluid chromatography (SFC) analysis that favours the chromatographic behaviours related to the stationary phase properties, bonding density, ligand type (monomeric or polymeric), and endcapping treatment, two separation factors are calculated allowing us to build a two-dimensional map. These two axes are related either to the shape selectivity or the polar surface activity (residual silanols). Each point on the map corresponds to a column. The retention factor of β-carotene, which describes the phase hydrophobicity, is indicated by the size of the point. More than 200 stationary phases were studied, including small particle sizes and superficially porous ones.