A Simple and Interactive Column Classification for Reversed-Phase Liquid Chromatography: The Carotenoid Test, Part I: Studied Properties, Probes, and Fundamentals
Cartenoid compounds can be used as probes for studying the stationary bonded phases devoted for reversed-phase liquid chromatography, that is, C18, phenyl-hexyl, and cholester. From one analysis achieved in supercritical fluid chromatography (SFC) that favors the chromatographic behaviors due to the stationary phase properties, bonding density, ligand type (monomeric or polymeric), and endcapping treatment, two separation factors are calculated allowing us to build a bi-dimentional map. These two axes are related either to the shape selectivity or the polar surface activity (residual silalnos). Each point on the map corresponds to a column. The retention factor of beta-carotene, which describes the phase hydrophobicity, is indicated by the size of the point. More than 200 stationary phases were studied, including small particle sizes and superficially porous ones. Moreover, the results are now available on a website, allowing you to check and compare, by selecting the required tabs, columns, manufacturer brands, and ligand nature.