LCGC Europe-10-01-2017

In his final column before retirement, John Dolan reminisces about his years as the author of “LC Troubleshooting”, and some of the changes that have taken place in liquid chromatography (LC) technology during that time.

In this study, an empirical assessment of summation integration (using the lowest baseline point in user-defined tR windows) was conducted involving 490 low-pressure GC–MS/MS analyses of 70 pesticides in 10 common fruits and vegetables over the course of 10 days.

Separating proteins and antibodies is a unique analytical challenge because of the complexity of the analytes. Both sample heterogeneity, because of a number of chemical modifications to the analyte, and nonspecific binding to the silica surface often result in chromatographic peak broadening and tailing. This instalment of “Column Watch” focuses on several different chromatographic strategies that analytical scientists can use to resolve and quantitate various biomacromolecules, including monoclobal antibodies (mAbs) and antibody–drug conjugates (ADCs). Selected aspects of size-exclusion, hydrophobic interaction, ion-exchange, hydrophilic interaction, and reversed-phase chromatography are discussed.

This instalment on ultrahigh-pressure liquid chromatography (UHPLC) reviews the potential problems that may be encountered using UHPLC systems and methods, and proposes strategies for their mitigation.

The Petroleum, Refining, and Environmental Monitoring Technologies Conference (PEFTEC 2017) will be held in the Antwerp Expo, Antwerp, Belgium, from 29–30 November 2017.

Click the title above to open the LCGC Europe October 2017 regular issue, Vol 30, No 10, in an interactive PDF format.