This week's Chromatography Online content spans a wide range of chromatography applications across health, food safety, and environmental science. A multinational team introduced a greener, miniaturized CPME-HPLC-DAD method for detecting four tetracyclines in urine, offering a simpler and cheaper alternative to LC-MS/MS, while our Associate Editorial Director Kate Jones also continued her conversation with Marinella Vitulli on untargeted HRMS screening for PFAS, bisphenols, and other emerging contaminants in food contact materials, including strategies for avoiding false positives in NIAS testing. On the clinical front, LC-MS/MS studies uncovered inflammatory lipid markers tied to recurrent pregnancy loss and linked urinary cotinine levels to tobacco exposure in Saudi women, while GC-MS combined with machine learning improved bladder cancer detection through urine testing. Elsewhere, new biosensors are being paired with chromatography for cannabinoid pain-therapy monitoring, GC/LC-MS methods examined how chlorine-radical treatments affect pesticide residues on strawberries, and HPLC-MS blood analysis identified linoleic acid and bile acids as potential markers of coronary artery disease risk.

This is the Best of the Week.

CPME-HPLC Method Detects Four Tetracyclines in Urine

A multinational research team developed a green, miniaturized CPME-HPLC-DAD method to simultaneously extract and detect four tetracyclines in human urine, offering a simpler, cheaper alternative to LC-MS/MS with improved sensitivity. LCGC International spoke to Fedra Vasileiou, Victoria Samanidou, and Abuzar Kabir, three of the authors of the paper that resulted from this work.¹

Untargeted HRMS Screening of Emerging Contaminants in Food Contact Materials: PFAS, Bisphenols, and NIAS
LCGC International presented the rest of our discussion with Marinella Vitulli—founder of Food Contact Center and Food Contact Services—about her work and her presentation at the recent ASMS conference titled, "Untargeted HRMS Screening of Emerging Contaminants in Food Contact Materials: PFAS, Bisphenols, and NIAS.” The talk detailed a suspect and non-target HRMS screening workflow built around proprietary spectral libraries for food contact material (FCM) safety assessment. ^2,3

Quality Control to Rule Out False-Positive NIAS Findings

The Biggest Gap in NIAS Testing for Food Contact Materials

LC-MS/MS Uncovers Lipid Clues to Recurrent Pregnancy Loss

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) reveals inflammatory lipid markers in recurrent pregnancy loss.⁴

GC-MS Urine Analysis Detects Bladder Cancer

Machine learning boosts gas chromatography-mass spectrometry (GC-MS) urine testing for bladder cancer detection.⁵

LC-MS/MS Tracks Cotinine in Saudi Women

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of urinary cotinine objectively assesses tobacco exposure in women.⁶

Detecting Cannabinoids: From GC-MS to Biosensors

Chromatography meets new biosensors for cannabinoid pain-therapy tracking.⁷

Chromatography Tracks Pesticides on Strawberries

Gas chromatography/liquid chromatography-mass spectrometry (GC/LC-MS) methods measure pesticide residue after chlorine-radical treatment.⁸

HPLC-MS Reveals Coronary Artery Disease Risk Markers
High-performance liquid chromatography-mass spectrometry (HPLC-MS) blood analysis links linoleic acid, bile acids to cardiac risk.⁹

References

1. Vasileiou, F.; Samanidou, V.; Kabir, A. et al. CPME-HPLC Method Detects Four Tetracyclines in Urine. Chromatography Online website.https://www.chromatographyonline.com/view/cpme-hplc-method-detects-four-tetracyclines-urine (accessed 2026-08-20)
2. Vitulli, M.; Jones, K. Quality Control to Rule Out False-Positive NIAS Findings. Chromatography Online website. https://www.chromatographyonline.com/view/quality-control-to-rule-out-false-positive-nias-findings (accessed 2026-08-20)
3. Vitulli, M.; Jones, K. The Biggest Gap in NIAS Testing for Food Contact Materials. Chromatography Online website. https://www.chromatographyonline.com/view/the-biggest-gap-in-nias-testing-for-food-contact-materials (accessed 2026-08-20)
4. Chasse, J. LC-MS/MS Uncovers Lipid Clues to Recurrent Pregnancy Loss.Chromatography Online website. https://www.chromatographyonline.com/view/lc-ms-ms-uncovers-lipid-clues-recurrent-pregnancy-loss (accessed 2026-08-20)
5. Chasse, J. GC-MS Urine Analysis Detects Bladder Cancer. Chromatography Online website. https://www.chromatographyonline.com/view/gc-ms-urine-analysis-detects-bladder-cancer(accessed 2026-08-20)
6. Chasse, J. LC-MS/MS Tracks Cotinine in Saudi Women.Chromatography Online website. https://www.chromatographyonline.com/view/lc-ms-ms-tracks-cotinine-saudi-women (accessed 2026-08-20)
7. Chasse, J. Detecting Cannabinoids: From GC-MS to Biosensors. Chromatography Online website. https://www.chromatographyonline.com/view/detecting-cannabinoids-gc-ms-to-biosensors (accessed 2026-08-20)
8. Chasse, J. Chromatography Tracks Pesticides on Strawberries. Chromatography Online website. https://www.chromatographyonline.com/view/chromatography-tracks-pesticides-strawberries(accessed 2026-08-20)
9. Chasse, J. HPLC-MS Reveals Coronary Artery Disease Risk Markers. Chromatography Online website. https://www.chromatographyonline.com/view/hplc-ms-reveals-coronary-artery-disease-risk-markers (accessed 2026-08-20)