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LCGC Europe-12-01-2017

LCGC Europe

The Use of Extraction Technologies in Food Safety Studies

December 01, 2017

Sample Preparation Perspectives

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12

Traditional extraction methods for food samples, such as liquid–liquid extraction and Soxhlet extraction, are often time-consuming and require large amounts of organic solvents. Therefore, one of the objectives of analytical food safety studies currently has been the development of new extraction techniques that can improve the accuracy and precision of analytical results and simplify the analytical procedure.

Peak Shapes and Their Measurements: The Need and the Concept Behind Total Peak Shape Analysis

December 01, 2017

Column Watch

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Gaussian peak shapes in chromatography are indicative of a well-behaved system. Such peak shapes are highly desirable from the perspective of column packing technology. From an analyst’s point of view, Gaussian peaks provide improved sensitivity (lower detection limits) and allow ease of quantitation. In practice, one can obtain peaks that tail, front, or concurrently front and tail for reasons such as column packing issues, chemical and kinetic effects, and suboptimal high performance liquid chromatography (HPLC) system plumbing and detector settings. Here, we discuss a number of approaches for peak shape measurement that are available in modern chromatography software, along with their advantages and drawbacks. A new “total peak shape analysis” approach is suggested that facilitates detection and quantification of concurrent fronting and tailing in peaks. Several remediation approaches are proposed that can help chromatographers analyze and improve peak shapes.

Resolving Volume-Restricted Metabolomics Using Sheathless Capillary Electrophoresis– Mass Spectrometry

December 01, 2017

Cover Story

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The analytical toolbox used in present-day metabolomics encounters difficulties for the analysis of limited amounts of biological samples. Therefore, a significant number of crucial biomedical and clinical questions cannot be addressed by the current metabolomics approach. Capillary electrophoresis–mass spectrometry (CE–MS) has shown considerable potential for the profiling of polar and charged metabolites in volume-restricted or mass-limited biological samples. This article considers advances that significantly improved the performance of CE–MS for in-depth metabolic profiling of limited sample amounts. Attention is also devoted to various technical aspects that still need to be addressed to make CE–MS a viable approach for volume-restricted metabolomics.

Vol 30 No 12 LCGC Europe December 2017 Regular Issue PDF

December 01, 2017

Issue PDF

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Click the title above to open the LCGC Europe December 2017 regular issue, Vol 30, No 12, in an interactive PDF format.