Ultrahigh-performance liquid chromatography–high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) reveals a chemical imbalance behind alcohol-positive milk.
"Perspectives in HPLC" examines 5 long-held HPLC "rules" (mobile phase filtration, routine buffer use, avoiding pH near an analyte's pKa, defaulting to 4.6-mm columns, and relying on AI for early method development), showing with experimental evidence that each is conditional rather than universally true in modern small-molecule drug testing.
In this ninth part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor explores the reasons why mobile phase filtration is not needed.
ChromSoc’s Data Analytics and AI in Chromatography symposium will debate whether AI could ever replace chromatographers, alongside pharma case studies.