To characterize biopharmaceuticals, particularly monoclonal antibodies and antibody–drug conjugates (ADCs), you need a complete toolbox of powerful tools. You are probably familiar with LC–MS methods. But have you seen what light-scattering detection can do?
Modern HPLC Strategies: Improving Retention and Peak Shape for Basic Analytes
August 16th 2024In high-performance liquid chromatography (HPLC), it is common for bases and unreacted ionized silanols on silica-based columns to cause irreproducible retention, broad peaks, and peak tailing when working with basic analytes. David S. Bell, Lead Consultant at ASKkPrime LLC offers innovative HPLC strategies that can help mitigate such issues.