Wyatt Technology Corporation | Authors

In this application note, two commercially available samples were analyzed in the ASTRA® software by SEC coupled to a multi-angle light scattering (MALS) detector (DAWN®), a refractive index detector (Optilab®), and a differential viscosity detector (ViscoStar®).

There has been a significant resurgence in the development of antibody-drug conjugates (ADC) as target-directed therapeutic agents for cancer treatment. Among the factors critical to effective ADC design is the Drug Antibody Ratio (DAR). The DAR describes the degree of drug addition that directly impacts both potency and potential toxicity of the therapeutic, and can have significant effects on properties such as stability and aggregation. Determination of DAR is, therefore, of critical importance in the development of novel ADC therapeutics.