LCGC UST
|Articles|February 7, 2011

Analysing dioxins in animal feed

In response to the recent discovery of dangerous levels of dioxins in animal feed produced in Germany, Phenomenex has announced its publication of a method for analysing the chemicals using high-resolution gas chromatography with mass spectrometric detection.

In response to the recent discovery of dangerous levels of dioxins in animal feed produced in Germany, Phenomenex has announced its publication of a method for analysing the chemicals using high-resolution gas chromatography with mass spectrometric detection (HRGC–MS).

Dioxins enter the environment and subsequently the food chain through combustion of organic materials in waste incineration. Recently, fat incorporated into feed products in Germany were found to be highly contaminated, and were reported to threaten a chain-reaction through the food supply. As a result several countries banned imports of animal products from Germany as a precautionary measure.

“While dioxin contamination is generally on the decline due to aggressive regulatory action, we can still see accidents, such as the one in Germany,” explains Sky Countryman, manager of applications and applied technology for the company. “Robust analytical testing procedures are still in demand for the determination of potential dioxin threats in both feed and animal fat.”

The method, developed in collaboration with Vista Analytical, also analyses dioxin-like compounds dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs), which are often monitored in conjunction with dioxins to give an overall toxic equivalent (TEQ) for a sample. The method has also been used to analyse non-organic chicken feed as well as fish from the Mississippi River and Great Lakes and shrimp from a variety of international sources.

The technical note on the HRGC–MS method can be accessed at www.phenomenex.com/dioxin.


Related to this article

Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.
Automated GC-MS/MS Method Tackles Oil Phthalates
Malte Hübschen of the University of Duisburg-Essen (Germany) discusses an automated gas chromatography-tandem mass spectrometry (GC-MS/MS) workflow that improves phthalate detection in tricky oils with LCGC International.
Best of the Week: From Olive Waste to mRNA Vaccines
This week's Chromatography Online highlights include olive waste antioxidant recovery, food and tea analysis, EV isolation, mRNA vaccine purification, perfusion chromatography for oligonucleotides, mass spectrometry in teaching and career advice from “Women in Chromatography.”