Breakthroughs in Simultaneous Liquid Chromatography Methods for Antioxidative Molecules


A recent study examined using liquid chromatography methods for determining analytes in antioxidative molecules.

Researcher Liliya Logoyda and her team from Horbachevsky Ternopil National Medical University in Ukraine, investigated creating simultaneous liquid chromatography (LC) analytical methods for specific antioxidative molecules (1). Their study, published in the Journal of Separation Science, explored antioxidative molecules such as resveratrol, tocopherol, and coenzyme Q10 in capsules (1).

Logoyda’s study examined a wide array of LC techniques, spanning the entire polarity spectrum (1). Various column matrixes, including Alkyl C18 bonded reversed phase, phenyl, cyanopropyl, diol, and the most polar base silica-filled column matrixes, were rigorously tested and recorded during the experiments (1). The study aimed to systematically compare and summarize the efficacy of these techniques in developing analytical methods for the three antioxidants (1).

The results achieved showed how the LC techniques that were investigated can determine the target group of analytes. The success of these methods hinged on meticulous analyses of molecular physicochemical data, particularly organic solubilities (1). Understanding the UV spectral absorption characteristics of each analyte was vital for this study. Knowing the constituents of the mobile phase was also important because it determined whether optimal separation could be achieved (1).

Key objectives included the development of methods using the isocratic mode of separation to yield symmetrical peak shapes, ensuring the highest sensitivity and accuracy, the shortest run time, and superior reproducibility (1). Examining the three antioxidants, the study found that they played a crucial role in successful method development. Factors such as organic solvent and water solubility, molecular polarities, and ultraviolet-absorption characteristics, including spectra and absorptivities, were all considered in overcoming challenges in rapid LC method development for simultaneous determination (1).

Logoyda and her team showed in this study how LC could be used to create more efficient and accurate methods for determining antioxidative molecules in capsules. The findings open new avenues for researchers and analysts seeking to refine simultaneous LC techniques, marking a significant stride towards enhanced precision and effectiveness in antioxidant analysis (1).

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(1) Piponski, M.; Topkoska-Naumoska, M.; Slaveska-Spirevska, I.; et al. Concepts and Principles for New Rapid Simple Liquid Chromatography Method for Quantification of Antioxidants Resveratrol, Vitamin E, and Coenzyme Q10 in Capsules with High-performance Liquid Chromatography with a Photo-diode Array Detector. J. Sep. Sci. 2023, ASAP. DOI: 10.1002/jssc.202300585

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