Researchers from Uppsala University (Uppsala, Sweden) added alkali salts to protein samples in preparation for capillary electrophoresis (CE) analysis of intact proteins.
Researchers from Uppsala University (Uppsala, Sweden) added alkali salts to protein samples in preparation for capillary electrophoresis (CE) analysis of intact proteins. The alkali salts were found to cause a high degree of peak stacking, even with large proteins. The salt addition reportedly promoted a strong improvement in the peak efficiency of individual proteins. The salt concentration required for the greatest peak efficiency was dependent on the molecular weight and molar concentration of the protein. The stacking effect was achieved using various surface-modified silica capillaries, which indicated that the phenomenon was not surface-coating specific.
