A Critical Evaluation of Hydrophilic-Interaction Chromatography (HILIC)
A Critical Evaluation of Hydrophilic-Interaction Chromatography (HILIC)
There are of course number of advantages related to HILIC, as listed in slide 4. The first one is that HILIC will allow sufficient retention of the most hydrophilic compound that does not possess sufficient retention under reversed-phase LC condition. The elution order will be almost reversed compared to reversed-phase LC, but it can also provide orthogonal selectivity and we will discuss some examples later. The very important advantage is MS sensitivity - it can be drastically improved because of a better desolvation of the mobile phase, which contains a huge amount of acetonitrile. Another important feature is that the back pressure remains very low in the HILIC mode because of the high proportion of organic modifier, which enables faster or high-resolution analysis. Finally, another advantage is the possibility to directly analyze solid-phase extraction (SPE) extracts in HILIC, because at the end of the SPE process, a huge amount of organic modifiers are present that are not critical for HILIC experiments. Van Deemter curves are also flatter when using a high proportion of organic solvent.
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The 26th Norwegian Symposium on Chromatography
March 29th 2024The 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.
Adjustable Polyacrylamide Stationary Phases Enhance Separation Performance in Liquid Chromatography
June 12th 2023Adjustable polyacrylamide stationary phases developed by researchers enhance separation performance in liquid chromatography, according to a recent publication. The study demonstrates the synthesis of vinyl modified silica and the successful preparation of polyacrylamide modified silica (PAM-SIL) stationary phases, allowing for fine control of separation performance.
An Interview with AI About Its Potential Role in Separation Science
May 17th 2023We interviewed an AI program (ChatGPT) for LCGC North America asking questions about AI and its role in various applications for separation science to include data analysis, and high performance liquid chromatography (HPLC), hydrophilic-interaction chromatography (HILIC), reversed-phase liquid chromatography (RPLC), liquid chromatography–mass spectrometry (LC–MS), gas chromatography–mass spectrometry (GC–MS), high resolution mass spectrometry (HRIM–MS), high resolution tandem mass spectrometry (HRMS/MS), and related topics.
