Detecting Nitric Oxide with Capillary Electrophoresis
Researchers from the Second Military Medical University (Shanghai, China) used capillary electrophoresis with laser-induced fluorescence detection to detect the release of nitric oxide from single neuron and mammalian cells.
Researchers from the Second Military Medical University (Shanghai, China) used capillary electrophoresis with laser-induced fluorescence detection to detect the release of nitric oxide from single neuron and mammalian cells. They used 8-(3,4-diaminophenyl)2,6-bis(2-carboxyethyl)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene as the fluorescent probe. They studied the feasibility of applying the method to four single-cell models. The method could be a powerful tool for studying the kinetics of nitric oxide release by neuronal cells during neurotransmission and for examining the pathobiological and therapeutic basis of nitric oxide for cardiovascular diseases.
Fundamentals of Benchtop GC–MS Data Analysis and Terminology
April 5th 2025In this installment, we will review the fundamental terminology and data analysis principles in benchtop GC–MS. We will compare the three modes of analysis—full scan, extracted ion chromatograms, and selected ion monitoring—and see how each is used for quantitative and quantitative analysis.
Characterizing Plant Polysaccharides Using Size-Exclusion Chromatography
April 4th 2025With green chemistry becoming more standardized, Leena Pitkänen of Aalto University analyzed how useful size-exclusion chromatography (SEC) and asymmetric flow field-flow fractionation (AF4) could be in characterizing plant polysaccharides.
This information is supplementary to the article “Accelerating Monoclonal Antibody Quality Control: The Role of LC–MS in Upstream Bioprocessing”, which was published in the May 2025 issue of Current Trends in Mass Spectrometry.
