Researchers from the Second Military Medical University (Shanghai, China) used capillary electrophoresis with laser-induced fluorescence detection to detect the release of nitric oxide from single neuron and mammalian cells.
Researchers from the Second Military Medical University (Shanghai, China) used capillary electrophoresis with laser-induced fluorescence detection to detect the release of nitric oxide from single neuron and mammalian cells. They used 8-(3,4-diaminophenyl)2,6-bis(2-carboxyethyl)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene as the fluorescent probe. They studied the feasibility of applying the method to four single-cell models. The method could be a powerful tool for studying the kinetics of nitric oxide release by neuronal cells during neurotransmission and for examining the pathobiological and therapeutic basis of nitric oxide for cardiovascular diseases.
LC–MS/MS-Based System Used to Profile Ceramide Reactions to Diseases
April 26th 2024Scientists from the University of Córdoba in Córdoba, Spain recently used liquid chromatography–tandem mass spectrometry (LC–MS/MS) to comprehensively profile human ceramides to determine their reactions to diseases.
High-Throughput 4D TIMS Method Accelerates Lipidomics Analysis
April 25th 2024Ultrahigh-pressure liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) had been previously proposed for untargeted lipidomics analysis, but this updated approach was reported by the authors to reduce run time to 4 min.