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Red wine is a challenging matrix that contains organic acids, sugars, phenols and pigments such as anthocyanins. This application note describes a simple, fast, novel and effective method to clean up red wine co-extractives.
a) Add 10 mL red wine to 50 mL tube (RFV0050CT)
b) Add 10 mL acetonitrile, vortex 30 s
c) Add salts from Mylar pouch (ECQUUS2-MP)
d) Shake vigorously for 1 min
e) Centrifuge at 5000 rpm for 5 min
f) Supernatant is ready for cleanup
a) Load 1 mL supernatant with disposable syringe
b) Push the supernatant slowly through the cartridge (ECPURMPSMC)
c) Collect 0.5 mL extract into 2 mL autosampler vial
d) Add triphenyl phosphate as internal standard
LC: Thermo Accela 1250 pump
MS-MS: Thermo TSQ Vantage
Recoveries ranged from 81.6 to 112.2% with RSD less than 10.8% achieved for red wine samples fortified with 10, 50 and 100 ng/mL pesticides.
Figure 1: Top: Quick QuEChERS cartridges before and after cleanup of 1 mL red wine extract; Bottom: Red wine extract before (left) and after (right) Quick QuEChERS cleanup.
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