Red wine is a challenging matrix that contains organic acids, sugars, phenols and pigments such as anthocyanins. This application note describes a simple, fast, novel and effective method to clean up red wine co-extractives.  

				b) Add 10 mL acetonitrile, vortex 30 s			

				e) Centrifuge at 5000 rpm for 5 min 			

				f) Supernatant is ready for cleanup			

				a) Load 1 mL supernatant with disposable syringe			

				b) Push the supernatant slowly through the cartridge 

c) Collect 0.5 mL extract into 2 mL autosampler vial 

				d) Add triphenyl phosphate as internal standard			

Column: SepaxHP-C18, 2.1 × 100 mm, 3 µm, 120 Å

Mobile phase: A: 0.1% formic acid in water; B: 0.1% formic acid in methanol

Gradient: 0 min: 95% A; 1 min: 95%; 3 min: 50% A; 8 min: 5% A; 14 min: 5% A; 14.2 min: 95% A; 16 min: 95% A

Recoveries ranged from 81.6 to 112.2% with RSD less than 10.8% achieved for red wine samples fortified with 10, 50 and 100 ng/mL pesticides. 

 Figure 1: Top: Quick QuEChERS cartridges before and after cleanup of 1 mL red wine extract; Bottom: Red wine extract before (left) and after (right) Quick QuEChERS cleanup.			

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