HPLC Analysis of Flavonoids in Ginkgo Biloba

Reseachers from China Pharmaceutical University (Nanjing, China) used high performance liquid chromatography (HPLC) with photodiode-array detection and electrospray ionization mass spectrometry to analyze 12 flavonoids in Ginkgo biloba leaves.

Reseachers from China Pharmaceutical University (Nanjing, China) used high performance liquid chromatography (HPLC) with photodiode-array detection and electrospray ionization mass spectrometry to analyze 12 flavonoids in Ginkgo biloba leaves. They also used chemiluminescence detection to determine the free radical scavenging activity of the flavonoids. They found that the luminescent signal could be inhibited by flavonol glycosides, which means that they are responsible for the leaves’ antioxidant activity. Flavonoids in plants have been reported to have protective effects against oxidative injury.