HPLC Determination of Artemisinin in Artemisia Annua L.

August 7, 2007

In an article recently published in Analytica Chimica Acta, researchers developed a procedure for the determination of artemisinin in A. annua using high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) in couple with microwave-assisted extraction (MAE) as an efficient sample preparation technique. Afterwards, method validation system for HPLC?ELSD analysis was developed.

In an article recently published in Analytica Chimica Acta, researchers developed a procedure for the determination of artemisinin in A. annua using high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) in couple with microwave-assisted extraction (MAE) as an efficient sample preparation technique. Afterwards, method validation system for HPLC–ELSD analysis was developed. Limit of detection was less than 40 É g mL-1 and limit of quantification was less than 100 É gmL-1.

Accuracy validation showed that average recovery was between 98.23% and 104.97%. The developed analytical procedure was successfully applied to determine the contents of artemisinin in the different parts of A. annua plants.

To view, please visit: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TF4-4KRNR39-8&_user=10&_coverDate=01%2F09%2F2007&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=b94b4f8ac6247105b7eac08804c3a65c