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HPLC Determination of Artemisinin in Artemisia Annua L.
In an article recently published in Analytica Chimica Acta, researchers developed a procedure for the determination of artemisinin in A. annua using high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) in couple with microwave-assisted extraction (MAE) as an efficient sample preparation technique. Afterwards, method validation system for HPLC?ELSD analysis was developed.
In an article recently published in Analytica Chimica Acta, researchers developed a procedure for the determination of artemisinin in A. annua using high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) in couple with microwave-assisted extraction (MAE) as an efficient sample preparation technique. Afterwards, method validation system for HPLC–ELSD analysis was developed. Limit of detection was less than 40 É g mL-1 and limit of quantification was less than 100 É gmL-1.
Accuracy validation showed that average recovery was between 98.23% and 104.97%. The developed analytical procedure was successfully applied to determine the contents of artemisinin in the different parts of A. annua plants.