Gas chromatography–mass spectrometry (GC–MS) and ion chromatography profile wasabi and mustard heat and acidity.
|Articles|September 1, 2008
Ion-Exchange Chromatography Protein Behavior
Scientists from Jacobs University (Bremen, Germany) used orthogonal proteomic methods to study the behavior of proteins in ion-exchange chromatography.
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Scientists from Jacobs University (Bremen, Germany) used orthogonal proteomic methods to study the behavior of proteins in ion-exchange chromatography. The researchers used a tailored protocol comprising a first separation based on isoelectric focusing with Immobiline strips under native conditions and second dimension based on an SDS-polyacrylamide gel electrophoresis run. The pI values obtained with the 2D electrophoresis procedure correlated with the ion-exchange chromatographic behavior. The proteins used in the study were thaumatin and bovine serum albumin.
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