Ion-Exchange Chromatography Protein Behavior
Scientists from Jacobs University (Bremen, Germany) used orthogonal proteomic methods to study the behavior of proteins in ion-exchange chromatography.
Scientists from Jacobs University (Bremen, Germany) used orthogonal proteomic methods to study the behavior of proteins in ion-exchange chromatography. The researchers used a tailored protocol comprising a first separation based on isoelectric focusing with Immobiline strips under native conditions and second dimension based on an SDS-polyacrylamide gel electrophoresis run. The pI values obtained with the 2D electrophoresis procedure correlated with the ion-exchange chromatographic behavior. The proteins used in the study were thaumatin and bovine serum albumin.
Determining Volatile Impurities and Ethanol Content in Hand Sanitizers with GC–MS
September 10th 2024Researchers analyzed 31 different brands of hand sanitizers using headspace gas chromatography-mass spectrometry (GC–MS) to assess volatile impurities and ethanol content in ethanol-based hand sanitizers.
Modern HPLC Strategies: Improving Retention and Peak Shape for Basic Analytes
August 16th 2024In high-performance liquid chromatography (HPLC), it is common for bases and unreacted ionized silanols on silica-based columns to cause irreproducible retention, broad peaks, and peak tailing when working with basic analytes. David S. Bell, Lead Consultant at ASKkPrime LLC offers innovative HPLC strategies that can help mitigate such issues.
