Ion-Exchange Chromatography Protein Behavior
Scientists from Jacobs University (Bremen, Germany) used orthogonal proteomic methods to study the behavior of proteins in ion-exchange chromatography.
Scientists from Jacobs University (Bremen, Germany) used orthogonal proteomic methods to study the behavior of proteins in ion-exchange chromatography. The researchers used a tailored protocol comprising a first separation based on isoelectric focusing with Immobiline strips under native conditions and second dimension based on an SDS-polyacrylamide gel electrophoresis run. The pI values obtained with the 2D electrophoresis procedure correlated with the ion-exchange chromatographic behavior. The proteins used in the study were thaumatin and bovine serum albumin.
Inside the Laboratory: The Chromatography Laboratory at the University of Rouen
April 18th 2024In this edition of “Inside the Laboratory,” Pascal Cardinael and Valérie Agasse of the University of Rouen in Mont‑Saint-Aignan, France, discuss their laboratory’s work with miniaturizing gas chromatography (GC) columns and systems to improve on-site air analysis of volatile organic compounds (VOCs).
