Ion-Exchange Chromatography Protein Behavior

Scientists from Jacobs University (Bremen, Germany) used orthogonal proteomic methods to study the behavior of proteins in ion-exchange chromatography. The researchers used a tailored protocol comprising a first separation based on isoelectric focusing with Immobiline strips under native conditions and second dimension based on an SDS-polyacrylamide gel electrophoresis run. The pI values obtained with the 2D electrophoresis procedure correlated with the ion-exchange chromatographic behavior. The proteins used in the study were thaumatin and bovine serum albumin.