Ion-Exchange Chromatography Protein Behavior
Scientists from Jacobs University (Bremen, Germany) used orthogonal proteomic methods to study the behavior of proteins in ion-exchange chromatography.
Scientists from Jacobs University (Bremen, Germany) used orthogonal proteomic methods to study the behavior of proteins in ion-exchange chromatography. The researchers used a tailored protocol comprising a first separation based on isoelectric focusing with Immobiline strips under native conditions and second dimension based on an SDS-polyacrylamide gel electrophoresis run. The pI values obtained with the 2D electrophoresis procedure correlated with the ion-exchange chromatographic behavior. The proteins used in the study were thaumatin and bovine serum albumin.
Evaluating the Accuracy of Mass Spectrometry Spectral Databases
May 12th 2025Mass spectrometry (MS) can be effective in identifying unknown compounds, though this can be complicated if spectra is outside of known databases. Researchers aimed to test MS databases using electron–ionization (EI)–MS.
