In high-performance liquid chromatography (HPLC), it is common for bases and unreacted ionized silanols on silica-based columns to cause irreproducible retention, broad peaks, and peak tailing when working with basic analytes. David S. Bell, Lead Consultant at ASKkPrime LLC offers innovative HPLC strategies that can help mitigate such issues.
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Gas chromatography–mass spectrometry (GC–MS) reveals a four-stage polycyclic aromatic hydrocarbons (PAH) breakdown in oil sludge co-composted with manure.
In this third part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor discusses the retention mechanism of hydrophobic interaction.
LCGC International spoke to David Hage, Sanduni Heenkenda, and Zoe Zingler of the University of Nebraska-Lincoln about how green chemistry principles are reshaping affinity chromatography.