Monday Morning Session Preview: Integrated Qualitative and Quantitative LC-MS for Small Molecule Analysis

June 5, 2013

E-Separation Solutions

The first talk in this session titled ?Integrated Quantitative and Qualitative work-flow for In-vivo Discovery Bioanalysis using Hybrid Quadrupole-Time-of-Flight Mass Spectrometry? will be given by Asoka Ranasinghe of Bristol-Myers Squibb Company.

Monday, 8:30 - 10:30 AM, Auditorium

The first talk in this session titled “Integrated Quantitative and Qualitative work-flow for In-vivo Discovery Bioanalysis using Hybrid Quadrupole-Time-of-Flight Mass Spectrometry” will be given by Asoka Ranasinghe of Bristol-Myers Squibb Company. Ranasinghe will discuss simultaneous in-vivo metabolic profiling and new chemical entities (NCE) quantification using data independent tandem mass spectrometry (MS-MS) and mass defect filter (MDF)-MS-MS, as well as dried blood spot (DBS) analysis of cyclosporine.

The second presentation, “Quantitative monitoring of tamoxifen extended to forty metabolites in human plasma using LC-HR-MS: New investigation capabilities for clinical pharmacology” by Bertrand Rochat of University Hospital of Lausanne, will discuss a new approach of tamoxifen dosage that includes 40 metabolites in patients’ plasma and shows new investigation capabilities for clinical pharmacology.

Jessica Albright of Northwestern University will present “A Quantitative LC–MS Method Allows Rapid Identification of Fungal Natural Products, their Biosynthetic Pathways, and Complex Mechanisms Regulating their Production” next. This talk is about the first large-scale mass spectrometry-based study to quantify thousands of small molecules produced by epigenetically-perturbed fungal systems.

The next talk will be given by Chad Satori of the University of Minnesota-Twin Cities and is titled “Workflow to Validate Compounds Identified in Enriched Autophagosome Fractions and Activated Mast Cell by UPLC-MSe.” Satori’s will discuss high confidence identification of compounds unique to enriched-autophagosome fractions and secretion from activated mast cells. It is believed that this work may be highly applicable to future metabolomic studies.

“Integrated High Throughput Quantitative and Qualitative Approaches for Pharmaceutical Research with HRMS (QqTOF)” is the next talk in this session, presented by Loren Olson of AB SCIEX. The focus will be on the evaluation of high speed automation with high resolution accurate mass analyzers for ADME quant and qual workflows.

The final presentation in this session, “Evaluation and integration of picoliter dispensing technology for LC-MS/MS analysis of small molecules in high throughput ADME and PK workflows,” will be given by Brian Furmanski of Siga Technologies. This talk is a bioanalytical assessment of a non-contact picoliter dispensing technology to aid in sample preparation of small molecules for LC–MS-MS analysis.