N-Glycan Separations: How Hydrophilic Interaction Liquid Chromatography (HILIC) Columns Affect Data Quality



Webinar Date/Time: Thursday, September 14th, 2023 at 8am PDT | 11am EDT | 4pm BST | 5pm CEST Thursday, September 14th, 2023 at 11am PDT | 2pm EDT | 7pm BST | 8pm CEST Friday, September 15th, 2023 at 11am CST | 12pm JST

Join us to learn about Agilent’s latest developmental HILIC column designed for maximum performance in N-glycan separations. In this webinar we’ll discuss how HILIC column selection plays a key role in generating useful N-glycan profile data when analyzing glycosylation of biotherapeutic glycoproteins. Data will also be presented which shows differences in separation performance among commercially available HILIC columns targeted at N-glycan analysis.

Register Free:

Event Overview.

Glycosylation can be a critical quality attribute (CQA) or product quality attribute (PQA) of biotherapeutic proteins, as the structure of N-linked glycans can influence protein function. Analysis of released N-glycans labeled with a dye that facilitates hydrophilic interaction liquid chromatography (HILIC) with detection by fluorescence (FLD) and/or mass spectrometry (MS) is a commonly used approach. The selection of an appropriate HILIC column for these separations is critical to ensure that glycans in complex mixtures are well-separated with minimal coelutions. Robustness in peak shape, retention time, and overall column lifetime are also often cited as important factors in choosing a suitable column.

This webinar will:

  • Present data on biotherapeutic molecules showing the N-glycan separation performance of Agilent’s latest developmental HILIC LC column
  • Provide comparisons of key N-glycan separations alongside other popular columns
  • Discuss key factors in column selection, such as selectivity, coelutions, peak shape, temperature stability, and longevity
  • Discuss how mobile phase composition can affect N-glycan charge group selectivity
  • Comparisons of MS and FLD response

Key Learning Objectives

  • Effects of column performance on N-glycan data analysis and data quality
  • Similarities and differences between HILIC LC columns from different manufacturers
  • Effects of mobile phase composition on N-glycan profile and separations

Who Should Attend

  • Biopharma analytical development scientists
  • Biopharma research and development scientists


Tom Rice
Product Manager, Bioconsumables
Agilent Technologies

Tom Rice is the Bioconsumables Product Manager at Agilent Technologies, coming to Agilent through the acquisition of ProZyme in 2018. Prior to joining Agilent, he held various sales and marketing roles within ProZyme, beginning in 2014. Much of Tom’s work both at ProZyme and Agilent has been focused on bringing innovative glycobiology solutions to biopharma customers. Tom is based in California and holds a BS from Sonoma State University.

Randall Robinson
Research and Development Scientist
Agilent Technologies

Randall Robinson is a Research and Development Scientist in the chemistries and supplies division at Agilent. Prior to joining Agilent in 2021, Randall completed his PhD in food chemistry with a focus on developing analytical techniques to characterize bioactive oligosaccharides milk and dairy streams. Since joining Agilent, he has contributed to the design of numerous products utilized for N-glycan analysis, including the HILIC column described in this presentation.

Register Free: https://www.chromatographyonline.com/lcgc_w/HILIC

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