Oligo to Go: Navigating your LC–MS Options for Oligonucleotide Characterization

Webcasts

Tuesday, June 28th, 2022 at 11am EDT|8am PDT|4pm BST|5pm CES This presentation will focus on a range of LC­MS options for the characterization of oligo samples and data analysis strategies behind those efforts. Specifically, oligo data from single quad and multiple high-resolution accurate-mass instruments will be explored.

Register Free: https://www.chromatographyonline.com/lcgc_w/oligo_char

Event Overview:

The comprehensive profiling of oligo products is critical and several attributes including intact mass, purity, and sequence are routinely determined by high-performance liquid chromatography–mass spectrometry (LC–MS). A variety of instrument and software options for these examinations exist and it can be challenging to navigate between them. This presentation will focus on a range of LC–MS options for the characterization of oligo samples and the data analysis strategies behind those efforts. Specifically, oligo data from single quadrupole and multiple high-resolution accurate-mass instruments will be explored. In addition, software workflows for intact mass determination, purity assessment, and sequence confirmation will be demonstrated.

Key Learning Objectives:

  • Describe and differentiate LC and MS hardware options for oligo measurements
  • Illustrate how commonly used hardware/software options accelerate research
  • Showcase how the Agilent BioConfirm 12 software supports automated oligo-target-plus-impurities workflows as well as oligo sequencing

Who Should Attend:

Scientists in analytical discovery, development, QA/QC, or manufacturing who use mass spectrometry to characterize oligonucleotides

For any technical questions please contact Jordan Ramesh: jramesh@mjhlifesciences.com

Speakers

Peter Rye, PhD
LCMS Applications Scientist
Agilent Technologies

Peter Rye is a pre-sales Application Engineer with Agilent Technologies. He has been with Agilent for 9 years developing LC, RapidFire, and mass spectrometry methods on a wide range of molecules. Prior to Agilent, Peter worked in analytical development groups for BioTove and BIOCIUS, the original companies that developed the RapidFire high-throughput MS platform.

Register Free: https://www.chromatographyonline.com/lcgc_w/oligo_char