Peak Integration that Matches the Efficiency Principles of UHPLC
UHPLC is fast becoming mainstream in many laboratories. With increased data, however, comes a directly proportional increase in data integration.
Ultra high performance liquid chromatography (UHPLC) technology is fast becoming mainstream in many laboratories. There are many compelling reasons for this switch, such as the speed of analysis, low solvent usage and significantly shorter runtimes resulting in many more samples being analysed.
With increased data, however, comes a directly proportional increase in data integration. Ensuring the accuracy and appropriateness of data integration is essential because it is used for all subsequent calculations, such as calibrations, determining the purity of drug substances and analysis of unknowns.
Optimizing a chromatogram’s integration continues to be a time-consuming task, even for the most experienced chromatographer and an area of significant resource drain for laboratories. It is mostly performed using a chromatographic data system (CDS) but while different software packages provide the means for the analyst to apply peak specific instructions to optimize integration, the process often involves trial and error methods or manual integration to determine the correct integration for each peak. Integration is also subjective and laboratories have different criteria and treatments. For example, not all chromatographers adopt the same approach when there are unresolved peak groups. Some might apply the valley-to-valley approach while others require exponential skim or a combination of these options.
Reversed-Phases for LC Deliberately Doped with Positive Charge: Tips and Tricks for Effective Use
May 13th 2025In this month's edition of LC Troubleshooting, Dwight Stoll and his fellow researchers discuss both the benefits (improved peak shape/loading) and challenges (excessive interaction) associated with charge-doped reversed-phase (RP) columns for both analytical and preparative separations.
Investigating 3D-Printable Stationary Phases in Liquid Chromatography
May 7th 20253D printing technology has potential in chromatography, but a major challenge is developing materials with both high porosity and robust mechanical properties. Recently, scientists compared the separation performances of eight different 3D printable stationary phases.
