This afternoon symposium focuses on opportunities to utilize compact capillary liquid chromatography (LC) techniques in the exploration of drug development. The session will be held in room 118A.

Milton Lee of Brigham Young University will open the session with a talk on the coming of age of compact capillary LC. Lee and his team have developed a hand-portable nanoflow LC instrument that has a small footprint, weighs only 5.4 kg, produces consistent nanoliter to milliliter flowrates at pressures up to 1500 bar, utilizes on-column or miniature flow cell optical UV detection, and is easy to operate.

Next, Samuel Foster of Rowan University will discuss kinetic profiling of capillary LC columns for use in compact LC instrumentation. Foster’s discussion focuses on an integrated column cartridge for use in a compact LC instrument that enables the installation of a wider variety of capillary-scale columns than was previously possible.

Peter Pellegrinelli of Advanced Materials Technology will then address opportunities for developing improved separation methods with modern ultrahigh-pressure LC (UHPLC) instruments. Examples will be shown for coupling a modern UHPLC instrument to new 1.5-mm i.d. columns. By reducing the column i.d. and length, along with making changes in particle size, a chromatographer can reduce solvent usage, decrease run times, and increase sensitivity, an important advantage for sample-limited applications.

Colleen Dugan of GSK will close the session with a presentation on HPLC method-development strategy (MDS) for small-molecule pharmaceutical analysis. In this presentation, the selection process for identifying eight columns to include in an updated MDS toolkit will be described.