Rapid and Accurate Simultaneous Quantitation of Low Concentration Amphetamines and Metabolites from Urine using Strata-X*-C and LC/MS/MS on Gemini® C18

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E-Separation Solutions

E-Separation SolutionsE-Separation Solutions-04-06-2009
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Use of Strata-X-C SPE cartridges allows for an aggressive organic wash of biological samples while maintaining high recoveries of amphetamines and their metabolites. In combination with LC/MS/MS, limits of quantitation (LOQ) of 0.54 ng/mL are achieved for MAMPH, HMMA, and MDMA and LOQ of 1.0 ng/mL are achieved for AMPH and MDA on Gemini C18 HPLC columns. Accuracy and reproducibility are not compromised with this simple and effective approach.

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