Reversed-Phase HPLC-MS Analysis of Salivary Peptides for Autism Diagnosis

January 15, 2009

Italian researchers in Rome (Universita Cattolica and Consiglio Nazionale delle Ricerche) and Cagliari (Universita di Cagliari) have used reversed-phase high performance liquid chromatography with electrospray ionization mass spectrometry to analyze naturally occurring salivary peptides from subjects with autism and compare the profiles with those obtained from age-matched control subjects.

Italian researchers in Rome (Universita Cattolica and Consiglio Nazionale delle Ricerche) and Cagliari (Universita di Cagliari) have used reversed-phase high performance liquid chromatography with electrospray ionization mass spectrometry to analyze naturally occurring salivary peptides from subjects with autism and compare the profiles with those obtained from age-matched control subjects. The phosphorylation level of four salivary phosphopeptides — statherin, histatin 1, and entire and truncated isoforms of acidic proline-rich proteins — was found to be lower in the autistic subjects. The researchers suggest that this analysis could help to determine a sizeable subgroup of autism spectrum disorder patients.