Separation of Benzimidazoles and Derivatives in Anti-Parasitic Drugs

September 1, 2016
Bingwen Yan, PhD, Kelly Johnson, ZirChrom Separations, Inc.

The Application Notebook

Issue 0

Page Number: 28



This multicomponent analysis of anti-parasitic drugs achieves baseline resolution of six closely related compounds in under 20 min using gradient elution and the unique selectivity of ZirChrom®-PBD.

Benzimidazoles are a large class of pharmaceuticals used in animal production with a broad spectrum of activity against roundworms (nematodes) (1). The longer half-life of oxfendazole and fenbendazole, due to their slow metabolization, allows these compounds to be more effective but also raises the concern of residues in the final food products. Here we present a gradient method of six closely related anti-parasitic pharmaceuticals.

Experimental

A mixture of six anti-parasitic pharmaceuticals was separated at 30 °C using a ZirChrom®-PBD column. Samples were 1 mg/mL and were in acetonitrile with the exception of benzthiazuron which was in methanol. The separation conditions were as follows:

This method can be tailored to your specific application needs. ZirChrom technical support can help to optimize and transfer this method to your site. Please contact ZirChrom technical support at 1-866-STABLE-1 or support@zirchrom.com for details.


Figure 1: Separation of six benzimidazoles.

ZirChrom phases offer unique selectivity, high efficiency, and excellent chemical and thermal stability.

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Reference

(1) http://www.merckvetmanual.com/mvm/pharmacology/anthelmintics/benzimidazoles.html

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