SPME of Polyphenols in Wine and Grapes

Researchers from the University of Murcia (Murcia, Spain) developed a sample pretreatment system based on solid-phase microextraction (SPME) followed by gas chromatography with mass spectrometry detection (GC–MS). They used a direct immersion SPME technique to adsorb the polyphenols and then placed the SPME fiber into the headspace of a BSTFA solution for derivatization for 15 min at 50 °C. They desorbed the analytes at 280 °C for 5 min. They were able to determine the analytes cis- and trans-resveratrol, piceatannol, catechin, and epicatechin in wine and grapes.