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Fundamental challenges in MS-based proteomics include the high sample complexity, the large dynamic range in protein concentration, and the resulting big-data computational analysis. Development in mass spectrometers towards higher sensitivity, faster sequencing speed and larger peak capacity address many of these challenges. With the introduction of the timsTOF platform the next evolution in technology is achieved by adding an additional dimension of gas-phase ion separation via the use of TIMS coupled to UHR-QTOF technology. This results in the transition from 3D-Proteomics (retention time, m/z and MS/MS fingerprint) into 4D-Proteomics™ (addition of intrinsic CCS values). 4D-Proteomics™ enabled by PASEF® allows scientists to dig deeper into their sample, provides more confidence in data, and increases data completeness by reducing the number of missing values. It is now possible to measure hundreds and thousands of omics samples due to the extremely high sequencing speed and proven robustness.
Benefits of the timsTOF platform
1. Increased Sensitivity and High Acquisition Speed at Ultra-High Resolution: PASEF enables MS/MS acquisition at >100 Hz, making high-throughput measurements using short gradients possible while maintaining deep proteome coverage or high protein depth measurements in less time. The time-focusing benefit of dual-TIMS funnel technology increases sensitivity thereby allowing for loading of lower sample amounts and simpler sample preparation techniques.
2. Mobility Offset Mass Aligned (MOMA): The MOMA benefit provided by TIMS technology identifies more near-isobaric peptides and post-translationally modified (PTMs) peptides per unit time. PTM-positional isomers, a common occurrence, are not only detected but can be quantified.
3. CCS-Aware Workflows and Open Data Format: CCS-Aware analysis software increases the confidence of identifications and quantitation, while increasing data completeness. Open data format easily allows timsTOF data to be used in custom developed bioinformatics solutions.
4. Increased Robustness:The orthogonal geometry of the inlet glass capillary to the axis of the mass analyzer combined with dual-TIMS funnel technology, greatly increases robustness, allowing for sustained MS performance over large sample cohorts, maximizing instrument uptime.
For research use only. Not for use in clinical diagnostic procedures.
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