Daniela Held | Authors


Tips & Tricks GPC/SEC Particle Size and the Effect of Mixing Columns of Different Particle Size

Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) columns are filled with porous particles differing in particle and pore sizes. Typical particle sizes in analytical GPC/SEC range from sub-2-µm particles applied in oligomer and protein separations to approximately 20 µm for separations of ultrahigh molar mass macromolecules (1,2). While the effect of combining columns of different pore sizes has previously been discussed in GPC/SEC Tips & Tricks (3,4), the effect of combining columns of different particle sizes has not been addressed before.

Tips & Tricks GPC/SEC: Silica Versus Polymer‑Based Columns

The chemistry of samples analyzed using gel permeation chromatography/size-exclusion chromatography/gel filtration chromatography (GPC/SEC/GFC) is very diverse. Different chemistries of stationary phases are required to allow for true size separation. Several types of materials are available, all of which have their advantages and limitations. While silica‑based stationary phases are most common in high performance liquid chromatography (HPLC), for macromolecules polymer-based phases are popular.

Tips & Tricks GPC/SEC: “Green” GPC/SEC and Solvent Alternatives

The “greenest solution” is certainly using no solvent but gel permeation chromatography/size-exclusion chromatography (GPC/SEC) as a liquid chromatography (LC) technique requires the use of a mobile phase. The growing awareness of the need for more sustainable (greener) solutions has focused attention on environmentally- and health-friendly solvents and solutions.