Wednesday Parallel Session: Capillary Electrophoresis and Liquid Chromatography–Mass Spectrometry (CE and LC–MS) of Proteins
The Wednesday Parallel Session will be hosted by Jonathan Sweedler of the University of Illinois at Urbana-Champaign. It will take place in the Golden Gate Ballroom C, floor B2 level.
The Wednesday Parallel Session will be hosted by Jonathan Sweedler of the University of Illinois at Urbana-Champaign. It will take place in the Golden Gate Ballroom C, floor B2 level
The first talk starts at 11:15 am with Kevin A. Schug of the University of Texas in Arlington, Texas presenting “Separation and Quantitation of Intact Proteins Using Liquid ChromatographyâTriple Quadrupole Mass Spectrometry.”
Next, Fangiun Wang of the Dalian Institute of Chemical Physics in Dalian, China will give a talk called “Specific Mixing Facilitates the Quantification of Protein Phosphorylation with Significant Dysregulation.”
Following Wang, Duygu Deniz Usta of Hacettepe University in Ankara Turkey will speak on “Phosphopeptide Enrichment Using Ti4+ Attached Monodisperse Porous Poly(phosphonic Acid)-Based Microspheres.”
Influence of Concentration in Conventional GPC/SEC and Advanced Detection GPC/SEC
March 21st 2025Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
