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In this fifth part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor discusses how to overcome challenges with reversed-phase chromatography in drug analysis.

Liquid chromatography–tandem mass spectrometry (LC–MS/MS) and microbiome data reveal kidney markers of diabetic disease.

Ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) shows Maresin1 drops in heatstroke and may protect the heart.

This week on Chromatography Online, leading experts shared practical insights with LCGC International on sustainable affinity methods, siRNA impurity profiling, nontarget screening, peptide tech transfer, and AI in separation science.

Liquid chromatography–mass spectrometry (LC–MS) was used to determine if there is a link between artificial sweeteners and breast cancer.

Chiral gas chromatography–mass spectrometry (GC–MS) shows how heat and storage alter linalool in blueberry juice.

Anneli Kruve reports that toxicity predictions from MS2-derived molecular fingerprints outperform spectral-similarity methods in nontarget screening of food.

In this fourth part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor explains why buffers are most often used in Mobile Phase A in high-pressure liquid chromatography of pharmaceuticals.