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© 2023 MJH Life Sciences and Chromatography Online. All rights reserved.
© 2023 MJH Life Sciences™ and Chromatography Online. All rights reserved.
Adriaan Ampe is with the Separation Science Group at Ghent University, in Ghent, Belgium.
Analyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.
In this first TRLC instalment, our recent developments, new possibilities, and current limitations of the use of 1D TRLC are discussed, while the column performance is described with respect to the fundamentals of HPLC.
TRLC can obtain separations similar to those of reversed-phase LC while using only water as the mobile phase.