U.S. EPA Method 531.2 describes the determination of widely used carbamate pesticides in raw surface water using HPLC with fluorescence detection following postcolumn derivatization, which enhances method sensitivity and selectivity as compared to UV absorbance detection.
Urea and allantoin are added to cosmetic products for skin protection and regeneration, especially for the treatment of dry skin, and analyzed for QC purposes. As polar compounds, they are not ideal for reversed-phase HPLC separations. Neutral hydrophilic compounds like urea and allantoin are best analyzed by hydrophilic interaction chromatography (HILIC). Traditional HILIC columns use silica modified with a hydrophilic group such as diol or cyano. Analytes are adsorbed and subsequently eluted with mobile phases containing high percentages of organic solvent (>75%).