Investigation of Reequilibration in Hydrophilic Interaction Liquid Chromatography
Interest in chromatography using hydrophilic interaction liquid chromatography (HILIC) has continued to build in recent years. Adoption of the technique has been slowed by experiences of poor reproducibility. In particular, re-equilibration times in HILIC have been reported as being exceptionally long as compared to reversed-phase chromatography. In this study, re-equilibration times in HILIC, for both aqueous–organic gradients and buffer gradients are systematically explored. The results not only promise to improve method development practices, but also provide insight into HILIC retention mechanisms across mechanistically differing polar stationary phases.