Screening and Characterizing Colloidal Interactions for Optimal Biotherapeutic Formulations
Colloidal interactions arising from surface-exposed moieties on therapeutic proteins, monoclonal antibodies, antibody–drug conjugates, and other biopharmaceuticals lie at the heart of drug product stability. Therefore, it is not surprising that much effort has been devoted to finding effective means to characterize these interactions and to rapidly screen drug candidates and formulations for optimal colloidal properties. The most common techniques for performing these analyses are based on analytical light scattering, in its two primary flavours: static light scattering (SLS) and dynamic light scattering (DLS). Recent advances in light scattering instrumentation, analytical methods, and algorithms provide developers of biologics with powerful tools to perform these studies.