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Evert-Jan Sneekes | Authors
Articles
LCGC North America
Nano LC: Principles, Evolution, and State-of-the-Art of the Technique
Nano LC is now widely applied in proteomics, and also is robust enough for application in fields such as pharmaceutical analysis.
LCGC Asia Pacific
Automated Off-Line Two-Dimensional LC–MS–MS of Complex Proteomic Samples with the UltiMate 3000 System
Multidimensional liquid chromatography (MDLC) techniques are essential for the separation of highly complex proteomic samples. Advantages of off-line MDLC techniques over on-line approaches include high flexibility in choice of column dimensions and mobile-phase compositions, and the ability to reanalyse sample fractions. Here we present a fully automated off-line two-dimensional chromatographic approach for the analysis of proteomic samples using an UltiMate 3000 system optimized for proteomics MDLC.